ABSTRACT
Keratinolytic microorganisms have become the subject of scientific interest due to their ability to biosynthesize specific keratinases and their prospective application in keratinic waste management. Among several bacterial classes, actinobacteria remain one of the most important sources of keratin-degrading strains, however members of the
Subject(s)
Animals , Keratins/metabolism , Micrococcus luteus/enzymology , Micrococcus luteus/metabolism , Peptide Hydrolases/metabolism , Biodegradation, Environmental , Chickens/microbiology , Feathers/microbiology , Micrococcus luteus/isolation & purification , NADH, NADPH Oxidoreductases/metabolism , Oxidation-Reduction , Poultry/microbiology , Sulfur Compounds/metabolism , Waste ManagementABSTRACT
High throughput macromolecular structure determination is very essential in structural genomics as the available number of sequence information far exceeds the number of available 3D structures. ACORN, a freely available resource in the CCP4 suite of programs is a comprehensive and efficient program for phasing in the determination of protein structures, when atomic resolution data are available. ACORN with the automatic model-building program ARP/wARP and refinement program REFMAC is a suitable combination for the high throughput structural genomics. ACORN can also be run with secondary structural elements like helices and sheets as inputs with high resolution data. In situations, where ACORN phasing is not sufficient for building the protein model, the fragments (incomplete model/dummy atoms) can again be used as a starting input. Iterative ACORN is proved to work efficiently in the subsequent model building stages in congerin (PDB-ID: lis3) and catalase (PDB-ID: 1gwe) for which models are available.